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The experimental design included three dietary protein levels (low, medium, high), three dietary rapamycin levels (control, 1 mg/kg, 5 mg/kg) and three fish per tank. The same diets were used throughout the feeding trial. The fish were fed three times per day at 12:00, 15:00 and 18:00 for a period of 12 weeks. Survival rates, weight gain, feed conversion ratios, specific growth rates, feed utilization, blood and tissue analyses were measured at different time points. The expression levels of TOR signaling related genes were measured in the liver, muscle and intestine of turbot. The results showed that:
Rapamycin significantly decreased the specific growth rates, feed conversion ratios and feed utilization in turbot.
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