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In vivo effects of doxycycline in a rabbit experimental model of chronic hepatitis B virus infection.
Doxycycline, a tetracycline derivative, is an effective drug in the treatment of chronic active hepatitis. The therapeutic benefit of doxycycline in hepatitis B virus (HBV)-related liver disease is, however, controversial. We evaluated the effect of doxycycline in a rabbit experimental model of chronic HBV infection. Twenty male rabbits were inoculated with 1 × 10(9) infectious units of a rNTCP-HBV chimeric virus expressing the NTCP receptor and infected for 1 month to establish a persistent HBV infection. Ten animals received, for 3 months, oral doxycycline 100 mg per day. Blood samples were taken periodically, and liver biopsies were performed before, and after 1, 2, and 3 months of doxycycline treatment. Liver tissue was evaluated histologically and immunohistochemically and quantitative PCR analysis of cccDNA was performed. Treatment with doxycycline was well tolerated. After 2 months of doxycycline treatment, cccDNA levels decreased significantly (p Post-exposure to maternally derived antibodies in a non-immune piglet.
The effects of passive transfer of maternally derived antibodies (MDA) in newborn pigs were studied in a cross-bred piglet. In a first experiment, piglets were given colostrum-free (control) or colostrum-treated (experiment 1) during the first hour after birth. All piglets were then injected 0b46394aab